The present study is aimed at investigating the subchronic toxicity of ethanol extract of Mucuna pruriens seeds from Indonesia. A total of 120 rats Wistar strain, both sexes, were used in this study. Both were divided into one control group, three treated groups, and two satellite groups. The extract in different doses was administered orally for 90 days for the treated groups, while 120 days for the satellite groups. The subchronic toxicity was evaluated using various parameters including death, motoric activity, body weight, haematology parameters, biochemical parameters, and organ weight along with the histopathological study. There was no mortality and no significant change in motoric activity, body weight, several haematological parameters, and glucose levels. Significant differences were found in levels of cholesterol, triglyceride, AST and ALT, BUN, and creatinine. Degeneration of some tissues was seen in the kidneys and the liver. The ethanol extract of Mucuna pruriens seeds from Indonesia has a reversible subchronic toxicity to the liver and kidneys.