PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

The aims of the present study are to isolate and characterise structure TLR3 and IL1β gene and evaluate the potential and signaling mechanism following exposure of Polyinosinic: polycytidylic acid (poly (I:C)), Lipopolysaccharide (LPS) and Zymosan as antigens in gouramy (Osphronemus gouramy). Gouramy kidney cells were stimulated with LPS, Poly I:C and Zymosan. Following incubation at 28°C, relative expression levels of Toll Like Receptor 3 (TLR3) and interleukin-1β (IL-1β) were examined at one hour and six hour after treatment. A Real Time Polimerase Chain Reaction approach was utilised to search for the effects of Poly I:C, LPS, and Zymosan exposure to gouramy kidney cells between one hour to six hours after treatment, LPS in kidney cell increased expression of interleukin-1β and downregulated expression of TLR3. Poly (I:C) which is an antigen that responds to antiviral, induces an increase in the transcription both of Toll like receptor 3 and IL-1β. Zymosan in kidney cells increased expression of TLR3 but downregulated expression of IL1β. This study shows that TLR3 was activated not only by Poly I: C but also LPS and Zymosan. However, antigen Poly IC-induced labor IL-1β and TLR3 expressed higher than the antigen LPS and Zymosan, so, this research showed a TLR3 response to Poly I:C is more dominant than Zymosan and LPS. TLR3 in gouramy kidney cell transcript was identified in response to poly I:C, Zymosan, and LPS. These results indicate that TLR3 in gouramy also plays a role for defense against bacterial infection and virus.