PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

 

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Comparative Clinicopathological Changes Associated with Experimental Streptococcus agalactiae and Streptococcus iniae Cohabitation Infection in Red Hybrid Tilapia (Oreochromis niloticus × Oreochromis mossambicus)

Salleh Annas, Mohd Zamri-Saad, Md Yasin Ina-Salwany and Mohammad Noor Azmai Amal

Pertanika Journal of Tropical Agricultural Science, Volume 46, Issue 3, August 2023

DOI: https://doi.org/10.47836/pjtas.46.3.10

Keywords: Streptococcus agalactiae, Streptococcus iniae, streptococcosis, tilapia

Published on: 30 August 2023

Streptococcus agalactiae and Streptococcus iniae are the two main pathogens causing streptococcosis in fish. This study compares the clinicopathological changes in red hybrid tilapia experimentally infected with S. agalactiae or S. iniae. A total of 180 tilapias were divided into six groups. Groups 1A, 2A, and 3A were inoculated intraperitoneally with sterile phosphate-buffered saline, S. agalactiae or S. iniae. Fish of Groups 1A, 2A, and 3A were then immediately allowed to cohabitate with fish of Groups 1B, 2B, and 3B, respectively. All fish were observed at 6-hr intervals for 120 hr before surviving fish were euthanized. The spleen, liver, and brain samples were collected for bacterial isolation and histopathology. Clinical signs were developed at 72 hr in Groups 2A and 3A and 96 hr in Groups 2B and 3B. Group 2A showed the highest clinical score (P<0.05). Significantly (P<0.05), more cohabitating fish (Groups 2B) were infected by S. agalactiae compared to S. iniae (Group 3B) at 55.0±0.0 and 43.70±1.25%, respectively. The mortality rate was significantly (P<0.05) higher for Groups 2A and 2B than other groups. The gross lesions were significantly (P<0.05) more common in fish of Group 2A. Histopathologically, encephalitis was observed in fish infected with S. iniae of Groups 3A and 3B, while meningoencephalitis was observed in fish infected with S. agalactiae of Groups 2A and 2B. The findings suggest that S. agalactiae is more pathogenic than S. iniae, producing slightly different histopathological lesions in the brain.

  • Abuseliana, A. F., Daud, H. H. M., Aziz, S. A., Bejo, S. K., & Alsaid M. (2011). Pathogenicity of Streptococcus agalactiae isolated from a fish farm in Selangor to juvenile red tilapia (Oreochromis sp.). Journal of Animal and Veterinary Advances, 10(7), 914-919. https://doi.org/10.3923/javaa.2011.914.919

  • Al-Harbi, A. H. (2016). Phenotypic and genotypic characterization of Streptococcus agalactiae isolated from hybrid tilapia (Oreochromis niloticus × O. aureus). Aquaculture, 464, 515-520. https://doi.org/10.1016/j.aquaculture.2016.07.036

  • Amal, M. N. A., & Zamri-Saad, M. (2011). Streptococcosis in tilapia (Oreochromis niloticus): A review. Pertanika Journal of Tropical Agricultural Science, 34(2), 195–206.

  • Amal, M. N. A., Zamri‐Saad, M., Iftikhar, A. R., Siti‐Zahrah, A, Aziel, S., & Fahmi, S. (2012). An outbreak of Streptococcus agalactiae infection in cage-cultured golden pompano, Trachinotus blochii (Lacépède), in Malaysia. Journal of Fish Diseases, 35(11), 849-852. https://doi.org/10.1111/j.1365-2761.2012.01443.x

  • Amal, M. N. A., Zamri-Saad, M., Siti-Zahrah, A., Zulkafli, R., Misri, S., Nur-Nazifah, M., & Shahidan, H. (2010). Prevalence of Streptococcus agalactiae in tilapia kept in different water bodies. Online Journal of Veterinary Research, 11(2),153-162.

  • Azzam-Sayuti, M., Ina-Salwany, M. Y., Zamri-Saad, M., Annas, S., Yusof, M. T., Monir, M. S., Mohamad, A., Muhamad-Sofie, M. H. N., Lee, J. Y., & Amal, M. N. A. (2021). Comparative pathogenicity of Aeromonas spp. in cultured red hybrid tilapia (Oreochromis niloticus × O. mossambicus). Biology, 10(11), 1192. https://doi.org/10.3390/biology10111192

  • Chen, C.-Y., Chao, C.-B., & Bowser, P. R. (2007). Comparative histopathology of Streptococcus iniae and Streptococcus agalactiae-infected tilapia. Bulletin of the European Association of Fish Pathologists, 27(1), 2–9.

  • Chen, M., Li, L.-P., Wang, R., Liang, W.-W., Huang, Y., Li, J., Lei, A.-I., Huang, W.-Y., & Gan, X. (2012). PCR detection and PFGE genotype analyses of streptococcal clinical isolates from tilapia in China. Veterinary Microbiology, 159(3-4), 526-530. https://doi.org/10.1016/j.vetmic.2012.04.035

  • Chu, C., Huang, P. Y., Chen, H. M., Wang, Y. H., Tsai, I. A., Lu, C. C., & Chen, C. C. (2016). Genetic and pathogenic difference between Streptococcus agalactiae serotype Ia fish and human isolates. BMC Microbiology, 16(1), 175. https://doi.org/10.1186/s12866-016-0794-4

  • Coureuil, M., Lécuyer, H., Bourdoulous, S., & Nassif, X. (2017). A journey into the brain: Insight into how bacterial pathogens cross blood–brain barriers. Nature Reviews Microbiology, 15, 149-159. https://doi.org/10.1038/nrmicro.2016.178

  • Eldar, A., Shapiro, O., Bejerano, Y., & Bercovier, H. (1995). Vaccination with whole-cell vaccine and bacterial protein extract protects tilapia against Streptococcus difficile meningoencephalitis. Vaccine, 13(9), 867-870. https://doi.org/10.1016/0264-410X(94)00067-W

  • Eto, S. F., Fernandes, D. C., de Moraes, A. C., da Costa Alecrim, J. V., de Souza, P. G., de Carvalho, F. C. A., Charlie-Silva, I., de Andrade Belo, M. A., & Pizauro, J. M. (2020). Meningitis caused by Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus): Infection and inflammatory response. Animals, 10(11), 2166. https://doi.org/10.3390/ani10112166

  • Evans, J. J., Pasnik, D. J., Klesius, P. H., & Al-Ablani, S. (2006). First report of Streptococcus agalactiae and Lactococcus garviae from wild bottlenose dolphin (Tursiops truncates). Journal of Wildlife Diseases, 42(3), 561-569. https://doi.org/10.7589/0090-3558-42.3.561

  • Ghozlan, A. A., Zaki, M. M., Gaber, M. M., & Nour, A. (2018). Effect of different water sources on survival rate (%) growth performance, feed utilization, fish yield, and economic evaluation on Nile tilapia (Oreochromis niloticus) monosex reared in earthen ponds. Oceanography and Fisheries Open Access Journal, 6(1), 555676. https://doi.org/10.19080/OFOAJ.2018.05.555676

  • Hoshina, T., Sano, T., & Marimoto, Y. (1958). A Streptococcus pathogenic to fish. Journal of Tokyo University Fisheries, 44, 57-58.

  • Jantrakajorn, S., Maisak, H., & Wongtavatchai, J. (2014). Comprehensive investigation of streptococcosis outbreaks in cultured Nile tilapia, Oreochromis niloticus, and red tilapia, Oreochromis sp., of Thailand. Journal of the World Aquaculture Society, 45(4), 392-402. https://doi.org/10.1111/jwas.12131

  • Klesius, P. H., Evans, J. J., & Shoemaker CA (2007). The macrophage chemotactic activity of Streptococcus agalactiae and Streptococcus iniae extracellular products (ECP). Fish and Shellfish Immunology, 22(5), 443-450. https://doi.org/10.1016/j.fsi.2006.05.001

  • Miao, W., & Wang, W. (2020). Trends of aquaculture production and trade: Carp, tilapia, and shrimp. Asian Fisheries Science, 33(S1), 1-10. https://doi.org/10.33997/j.afs.2020.33.s1.001

  • Mishra, A., Nam, G. H., Gim, J. A., Lee, H. E., Jo, A., & Kim, H. S. (2018). Current challenges of Streptococcus infection and effective molecular, cellular, and environmental control methods in aquaculture. Molecules and Cells, 41(6), 495-505. https://doi.org/10.14348/molcells.2018.2154

  • Nurliyana, M., Lukman, B., Ina-Salwany, M. Y., Zamri-Saad, M., Annas, S., Dong, H. T., Rodkum, C., & Amal, M. N. A (2020). First evidence of scale drop disease virus in farmed Asian seabass (Lates calcarifer) in Malaysia. Aquaculture, 528, 735600. https://doi.org/10.1016/j.aquaculture.2020.735600

  • Nur-Nazifah, M., Firdaus-Nawi, M., Sabri, M. Y., Siti-Zahrah, A., & Zamri-Saad, M. (2011). Determination of LD50 for Streptococcus agalactiae and Staphylococcus aureus infections in tilapia. Jurnal Veterinar Malaysia, 23(2), 22-27.

  • Poyart, C., Pellegrini, E., Gaillot, O., Boumaila, C., Baptista, M., & Trieu-Cuot, P. (2001). Contribution of Mn-cofactored superoxide dismutase (SodA) to virulence of Streptococcus agalactiae. Infection and Immunity, 69(8), 5098-5106. https://doi.org/10.1128/IAI.69.8.5098-5106.2001

  • Quagliarello, V., & Scheld, W. M. (1992). Bacterial meningitis: Pathogenesis, pathophysiology, and progress. The New England Journal of Medicine, 327(12), 864-872. https://doi.org/10.1056/NEJM199209173271208

  • Rahmatullah, M., Ariff, M., Kahieshesfandiari, M., Daud, H. M., Zamri-Saad, M., Sabri, M. Y., & Ina-Salwany, M. Y. (2017). Isolation and pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia. Journal of Aquatic Animal Health, 29(4), 208-213. https://doi.org/10.1080/08997659.2017.1360411

  • Song, Z., Ruichao, Y., Yanming, S., Chunfa, L., Sher, H. K., Chaosi, L., Ying, Z., Xiangmei, Z., Lifeng, Y., & Deming, Z. (2017). Fatal bacterial septicemia in a bottlenose dolphin Tursiops truncatus caused by Streptococcus iniae. Diseases of Aquatic Organisms, 122(3), 195-203. https://doi.org/10.3354/dao03069

  • Syuhada, R., Zamri-Saad, M., Ina-Salwany, M. Y., Mustafa, M., Nasruddin, N. N., Desa, M. N. M., Nordin, S. A., Barkham, T., & Amal, M. N. A. (2020). Molecular characterization and pathogenicity of Streptococcus agalactiae serotypes Ia ST7 and III ST283 isolated from cultured red hybrid tilapia in Malaysia. Aquaculture, 515, 734543. https://doi.org/10.1016/j.aquaculture.2019.734543

  • Tunkel, A. R., & Scheld, W. M. (1993). Pathogenesis and pathophysiology of bacterial meningitis. Clinical Microbiology Reviews, 6(2), 118-136. https://doi.org/10.1128/cmr.6.2.118

  • Ulum, M. T. Z., Ab Azid, M. A., Shen, T. W., Hassim, H. A., Zamri-Saad, M., & Salleh, A. (2021). Histopathological changes in the reproductive organs of does with pregnancy toxaemia and their aborted foetuses. Small Ruminant Research, 199, 106363. https://doi.org/10.1016/j.smallrumres.2021.106363

  • Yuasa, K., Kamaishi, T., Hatai, K., Bahnnan, M., & Borisutpeth, P. (2008). Two cases of streptococcal infections of cultured tilapia in Asia. http://www.fhs-afs.net/daa_vi_files/19.pdf

  • Zamri-Saad, M., Amal, M. N. A., & Siti-Zahrah, A. (2010). Pathological changes in red tilapias (Oreochromis spp.) naturally infected by Streptococcus agalactiae. Journal of Comparative Pathology, 143(2–3), 227–229. https://doi.org/10.1016/j.jcpa.2010.01.020

ISSN 1511-3701

e-ISSN 2231-8542

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JTAS-2641-2022

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