PERTANIKA JOURNAL OF SCIENCE AND TECHNOLOGY

Cabe Jawa (Piper retrofractum Vahl.) is categorised in Piper's genus under family piperaceae. It is commonly reported to function as antimicrobial, antifungal and anti-inflammatory. The aim of this study is to obtain information on the isolation of secondary metabolites from n-hexane extract of Cabe Jawa fruits. The isolation stage began with the maceration method with n-hexane solvent, then continued with purification stage of fractination compounds which was conducted by using several chromatography techniques including thin layer chromatography (TLC), vacuum liquid chromatography (VLC), and radial chromatography. Methyl piperate compound was isolated from this extract. The structure of this compound was determined using spectroscopic ¹H NMR. Based on the results of spectroscopic, analysis of 1H NMR was present at a chemical shift of 3.80 ppm singlet peak with integrity of 3H was the typical peak of a methoxy. At 6.12 ppm chemical shift indicated a signal peak for protons bound to C sp2. Chemical shift at 6.96 ppm to 7.13 ppm for proton groups was attached to benzene ring. From the results of ¹H NMR spectrum assisted by biogenesis approach, it is concluded that the purified compound successfully isolated was a methyl piperate compound.