PERTANIKA JOURNAL OF SCIENCE AND TECHNOLOGY

Collagen from the local ”Kacang” goat skin is a natural raw material in the halal food industry in Indonesia. This study aims to isolate acid-soluble collagen (ASC) from “Kacang” goat skin and characterize its chemical properties. The collagen was derived from one-year-old goat skin and cured in acid condition for 48 hours at 4°C to eliminate meats, fat, and hair. The cleaned skins were treated at 1:10 (w/v) of 0.1 M NaOH for 0, 24, and 48 h at 4°C. It was then neutralized in distilled water and extracted with 0.5 M acetic acid at a 1:10 (w/v) ratio for 24, 48, and 72 h at 4°C. The yield of ASC was 21%, characterized by chemical composition, soluble protein, differential scanning calorimeter (DSC), and protein molecular weight. The chemical composition of ASC was 11.15% (moisture), 9.04% (protein), 0.98% (fat), and 0.052% (ash). ASC has the highest collagen solubility in NaCl 5% at pH 2. ASC also has thermal stability with a low profile pattern of molecular weight. In conclusion, “Kacang” goat skin from Indonesia might be used to make a value-added product because it has a high moisture content and low fat level.

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