e-ISSN 2231-8526
ISSN 0128-7680
Athirah Mohd Bakri and Yuzine Esa
Pertanika Journal of Science & Technology, Volume 31, Issue 4, July 2023
DOI: https://doi.org/10.47836/pjst.31.4.18
Keywords: captive, CO1 mtDNA, genetic distance, L. calcarifer
Published on: 3 July 2023
This study examined the genetic diversity of Asian seabass (Lates calcarifer) captive populations using sequencing of the mitochondrial DNA cytochrome c oxidase I (COI) fragment. The phylogenetic analyses of the 609 base pair regions of the COI fragment from 146 samples identified 15 haplotypes and divided them into two clades with a genetic divergence of 10%. Thus, phylogenetic results supported two genetic groups (the Australia/Southeast Asia group and the India/Myanmar group) within the captive populations under study. Mixed levels of genetic diversity were observed among captive populations, which indicated a certain degree of inbreeding depression. The findings would be useful for future aquaculture management of captive Asian seabass in Malaysia.
Das, S. P., Swain, S., Jena, J., & Das, P. (2018). Genetic diversity and population structure of Cirrhinus mrigala revealed by mitochondrial ATPase 6 gene. Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis, 29(4), 495-500. https://doi.org/10.1080/24701394.2017.1310852
Falush, D., Stephens, M., & Pritchard, J. K. (2003). Inference of population structure using multilocus genotype data: Linked loci and correlated allele frequencies. Genetics, 164(4), 1567-1587. https://doi.org/10.1093/genetics/164.4.1567
Froese, R., & Pauly, D. (Eds.). (2022). FishBase. World Wide Web electronic publication. http://www.fishbase.org
Grant, W. S., & Bowen, B. W. (1998). Obituary: Professor Michael Laskowski Sr. Acta Biochimica Polonica, 30(2), 113-114.
Hebert, P. D. N., Cywinska, A., Ball, S. L., & DeWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270(1512), 313-321. https://doi.org/10.1098/rspb.2002.2218
Idris, S. M., Noordin, W. N. M., Manah, F. O., & Hamzah, A. (2022). Toward systematic breeding of asian sea bass, Lates calcarifer (Bloch, 1790), in Malaysia: status, challenges and prospects for future development. Asian Fisheries Science, 35(1), 1-12. https://doi.org/10.33997/j.afs.2022.35.1.001
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. https://doi.org/10.1093/molbev/msw054
Norfatimah, M. Y., Azizah, M. N. S., Othman, A. S., Patimah, I., & Jamsari, A. F. J. (2009). Genetic variation of Lates calcarifer in Peninsular Malaysia based on the cytochrome b gene. Aquaculture Research, 40(15), 1742-1749. https://doi.org/10.1111/j.1365-2109.2009.02279.x
Senanan, W., Pechsiri, J., Sonkaew, S., Na-Nakorn, U., Sean-In, N., & Yashiro, R. (2014). Genetic relatedness and differentiation of hatchery populations of Asian seabass (Lates calcarifer) (Bloch, 1790) broodstock in Thailand inferred from microsatellite genetic markers. Aquaculture Research, 46(12), 2897-2912. https://doi.org/10.1111/are.12442
Vij, S., Purushothaman, K., Gopikrishna, G., Lau, D., Saju, J. M., Shamsudheen, K. V., Vinaya Kumar, K., Basheer, V. S., Gopalakrishnan, A., Hossain, M. S., Sivasubbu, S., Scaria, V., Jena, J. K., Ponniah, A. G., & Orbán, L. (2014). Barcoding of Asian seabass across its geographic range provides evidence for its bifurcation into two distinct species. Frontiers in Marine Science, 1, Article 30. https://doi.org/10.3389/fmars.2014.00030
Ward, R. D., Holmes, B. H., & Yearsley, G. K. (2008). DNA barcoding reveals a likely second species of Asian sea bass (barramundi) (Lates calcarifer). Journal of Fish Biology, 72(2), 458-463. https://doi.org/10.1111/j.1095-8649.2007.01703.x
Ward, R. D, Zemlak, T. S., Innes, B. H., Last, P. R., & Hebert, P. D. N. (2005). DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462), 1847-1857. https://doi.org/10.1098/rstb.2005.1716
Zhang, Q., Sun, C., Zhu, Y., Xu, N., & Liu, H. (2020). Genetic diversity and structure of the round-tailed paradise fish (Macropodus ocellatus): Implications for population management. Global Ecology and Conservation, 21(159), Article e00876. https://doi.org/10.1016/j.gecco.2019.e00876
Zhu, Z. Y., Lin, G., Lo, L. C., Xu, Y. X., Feng, F., Chou, R., & Yue, G. H. (2006). Genetic analyses of Asian seabass stocks using novel polymorphic microsatellites. Aquaculture, 256(1-4), 167-173. https://doi.org/10.1016/j.aquaculture.2006.02.033
ISSN 0128-7680
e-ISSN 2231-8526