The aim of this study was to investigate the survival rates and the behavioural responses of Holothuria leucospilota under hypoxia stress condition. A total of 15 H. leucospilota were collected from Rambut Island, in Thousand Islands, Jakarta, Indonesia. They were exposed to three different dissolved oxygen concentrations (i.e. 2 mg O₂ L^-1 , 3 mg O₂ L^-1 , >6 mg O₂ L^-1 ). Mortality was measured at the 4th and 6th day by adding 2 mg O₂ L^-1 and 3 mg O₂ L^-1 of dissolved oxygen concentrations respectively. The survival rates of H. leucospilota significantly decreased by 80% at 2 mg O₂ L^-1 and 40% at 3 mg O₂ L^-1 compared to > 6 mg O₂ L^-1 in which total survival was 100% at the end of 22 days of exposure to hypoxia. The behavioural responses of H. leucospilota to hypoxia stress were shown by decreased metabolic activity by releasing the contents of their stomach described as cuvierian tubules. The lower dissolved oxygen concentrations at 2 mg O₂ L^-1 and 3 mg O₂ L^-1 showed a higher metabolic rate in 20% and 50 % of individual organisms during 18 days hypoxia exposure. If their metabolic rate is high, they will need more energy reserves to compensate it. The result of this study revealed that the lowest dissolved oxygen at 2 mg O₂ L^-1 can exert a stress on H. leucospilota which can lead to a high mortality and an impairment of behavioural responses.