PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

 

e-ISSN 2231-8542
ISSN 1511-3701

Home / Regular Issue / JTAS Vol. 41 (2) May. 2018 / JTAS-S0024-2018

 

Effect of Cytokinins on In Vitro Growth of Hypocotyl and Cotyledon of Tomato (Lycopersicon esculentum)

Wina Dian Savitri, Popy Hartatie Hardjo, Leonardo Tejo Gunawan Putra Hardianto and Steven Sutanto

Pertanika Journal of Tropical Agricultural Science, Volume 41, Issue 2, May 2018

Keywords: Cotyledon, cytokinins, hypocotyls, in vitro growth, tissue regeneration

Published on: 31 May 2018

Study of regeneration from different tissues or organs of plants is important as it gives information on how a piece of a plant can transform into its whole form. This process is even substantial when we talk about genetic engineering in plants, since no genetic engineering is valuable without knowing first the standard protocol for regenerating the transformed tissue or organs to become a whole plant. This experiment used hypocotyl and cotyledon of tomato cv. Tymoti as the explants was used to study how different concentrations (1.5-3 ppm) of cytokinins (Kinetin (Kin), 6-benzylaminopurine (BAP), thidiazuron (TDZ) and Zeatin (Zn)) affect its growth. As many as 16 explants were used for each treatment. The growth of both explants in the Murashige and Skoog (MS) media + vitamins showed that Zn and TDZ were superior among the other treatments in inducing calli and primordia organ.

ISSN 1511-3701

e-ISSN 2231-8542

Article ID

JTAS-S0024-2018

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