PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

Chloris gayana grass is a perennial tropical grass that is highly adaptable and resistant to climate change. As a cover crop, this grass can improve soil conditions. This grass has high palatability and potential to be improved through breeding to enhance the quality and productivity of ruminants’ feed. This research was conducted to determine the effect of gamma ray radiation on the morphology, productivity and genetic variation of C. gayana cv. Callide. This research used 4 radiation doses of 0, 75, 150 and 225 Gy, which were given to C. gayana cv Callide plant seeds via a Gamma 60Co irradiator chamber. A radiation dose of 150 Gy was proven to increase the development of culm diameter (p < 0.05). The best productivity was shown by radiation doses of 75 and 150 Gy, which increased the production of fresh, dry, and organic matter. The results of genetic variation analysis using Random Amplified Polymorphic Deoxyribonucleic Acid Polymerase Chain Reaction (RAPD PCR) showed that the polymorphism resulting from using the Organophosphate Degradation (OPD) 8 primer was 66.67%, and the OPD 11 primer was 62.50%. Overall, gamma ray radiation with 75 and 150 Gy doses on C. gayana cv. Callide has been proven to improve plant productivity.

• Abera, M. (2017). Biomass yield and biological potential of grass (Chloris gayana) and legume (Medicago sativa) mixtures under variable seed rates in Southern Region of Ethiopia. Greener Journal of Agricultural Sciences, 7(6), 132-136. https://doi.org/10.15580/gjas.2017.6.080817102.
  
  Allah, Y. N., & Bello, A. (2019). The potentials of rhodes Grass (Chloris gayana Kunth) as drought resistant perennial forage grass in Nigeria. American Journal of Biomedical Science & Research, 6(3), 188-194. https://doi.org/10.34297/ajbsr.2019.06.001025.
  
  Ananta, D., Bachruddin, Z., & Umami, N. (2019). Growth and production of 2 cultivars (Pennisetum purpureum Schumach.) on regrowth phase. IOP Conference Series: Earth and Environmental Science, 387, 012033. https://doi.org/10.1088/1755-1315/387/1/012033.
  
  Anggereini, E. (2008). Random amplified polymorphic DNA (RAPD), suatu metode analisis DNA dalam menjelaskan berbagai fenomena biologi [Random amplified polymorphic DNA analysis method and biological phenomena]. Biospecies, 1(2), 73-76.
  
  Association of Official Analytical Chemists. (2005). Official method of association of official analytical chemist. Benjamin Franklin Station.
  
  Badan Pusat Statistik. (2023). Populasi sapi perah menurut provinsi [Dairy cattle population by province]. Badan Pusat Statistik Jakarta. https://www.bps.go.id/id/statistics-table/2/NDcwIzI=/populasi-sapi-perah-menurut-provinsi.html.
  
  Beyaz, R., Kahramanogullari, C. T., Yildiz, C., Darcin, E. S., & Yildiz, M. (2016). The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss. under in vitro conditions. Journal of Environmental Radioactivity, 162-163, 129-133. https://doi.org/10.1016/j.jenvrad.2016.05.006.
  
  Beyaz, R., Ozgen, Y., Cavdar, A. & Yildiz, M. (2020). The effect of gamma radiation and magnetic field on seed germination and seedling growth at low temperature in sorghum x sudangrass hybrids. Maydica, 65, 1-6.
  
  Bilgin, F. D., & Tansi, V. (2020). Perennial warm season grasses; Cultivation of rhodes grass (Chlorois gayana L.) and dallisgrass (Paspalum dilatatum Poir.). In S. Seydosoglu (Ed.), Innovative approaches in meadow-rangeland and forage crops (pp. 177-195). Iksad Publishing.
  
  Ceccarelli, S. (2015). Efficiency of plant breeding. Crop Science, 55, 87-97. https://doi.org/10.2135/cropsci2014.02.0158.
  
  Ciocan, A.-G., Maximilian, C., Mitoi, E. M., Moldovan, R.-C., Neguț, D., Iuga, C.-A., Helepciuc, F. E., Holobiuc, I., Radu, M., Vassu Dimov, T., & Cogălniceanu, G. (2023). The impact of acute low-dose gamma irradiation on biomass accumulation and secondary metabolites production in Cotinus coggygria Scop. and Fragaria × ananassa Duch. red callus cultures. Metabolites, 13, 894. https://doi.org/10.3390/metabo13080894.
  
  Daba, A. W., Qureshi, A. S., & Nisaren, B. N. (2019). Evaluation of some rhodes grass (Chloris gayana) genotypes for their salt tolerance, biomass yield and nutrient composition. Applied Sciences, 9, 143. https://doi.org/10.3390/app9010143.
  
  Delastra, M. N., Astuti, A., Suwignyo, B., Muhlisin, M., & Umami, N. (2021). Gamma radiation effect on growth, production and lignin content of Sorghum sudanense at different harvest ages. Buletin Peternakan, 45(3), 183-188. https://doi.org/10.21059/buletinpeternak.v45i3.62627.
  
  Due, M. S., Susilowati, A., & Yunus, A. (2019). The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker. Biodiversitas, 20(5), 1416-1422. https://doi.org/10.13057/biodiv/d200534.
  
  Fahmi, M., Utomo, R., & Umami, N. (2019). Physical and chemical quality of silage from two Pennisetum purpureum sp varieties supplemented with molasses at different levels. IOP Conference Series: Earth and Environmental Science, 387, 012059. https://doi.org/10.1088/1755-1315/387/1/012059.
  
  Gusmiaty, Restu, M., Asrianny., & Larekeng, S. H. (2016). Polimorfisme penanda RAPD untuk analisis keragaman genetik Pinusmerkusii di hutan Pendidikan Unhas [RAPD marker polymorphism for genetic diversity analysis of Pinusmerkusii in the educational forest of Unhas]. Jurnal Natur Indonesia, 16(2), 47-53. https://doi.org/10.31258/jnat.16.2.47-53.
  
  Harmini, H., Sajimin, S., Fanindi, A., & Husni, A. (2021). Produktivitas rumput gajah (Pennisetum purpureum cv Taiwan) hasil irradiasi sinar gamma pada dosis 50 Gy [Productivity of elephant grass (Pennisetum purpureum cv Taiwan) from gamma ray irradiation at a dose of 50 Gy]. Jurnal Ilmu Peternakan Terapan, 5(1), 1-7. https://doi.org/10.25047/jipt.v5i1.2906.
  
  Hartati, D., Rimbawanto, A., Taryono, Sulistyaningsih, E., & Widyatmoko, A. Y. P. B. C. (2007). Pendugaan keragaman genetik di dalam dan antar provenan pulai (Alstonia scholaris (L.) R. Rr.) menggunakan penanda RAPD [Estimation of genetic diversity within and among pulai (Alstonia scholaris (L.) R. Rr.) provenance revealed by RAPD marker]. Jurnal Pemuliaan Tanaman Hutan, 1(2), 51-98. https://doi.org/https://doi.org: 10.20886/jpth.2007.1.2.89-98.
  
  Hartati, S., Prasetyo, P., & Muliawati, E. S. (2021). Effects of gamma irradiation on phenotypic changes in vanda hybrid. Journal of Biodiversity and Biotechnology, 1(1), 1-4. https://doi.org/10.20961/jbb.v1i1.49298.
  
  Hidosa, D., Hitiso, W., & Guyo, M. (2018). Biomass production of different grass species available at irrigated lowland of Dassench Woreda in South Western Ethiopia. Bangladesh Journal of Animal Science, 46(3), 188-191. https://doi.org/10.3329/bjas.v46i3.36313.
  
  Hoka, A. I., Gicheru, M., Otieno, S., & Korir, H. (2019). Effect of gamma irradiation of local Brachiaria ruziziensis (Germain & Evrard) seeds on agronomic performance and yield. Journal of Plant Breeding and Genetics, 7(1), 9-17. https://doi.org/10.33687/pbg.007.01.2836.
  
  Jabessa, T., Bekele, K., & Tesfaye, G. (2023). Evaluation of rhodes grass (Chloris gayana) cultivars for forage yield and yield components at highland and mid- land of Guji Zone Southern Oromia. Austin Journal of Nutrition & Metabolism, 10(1), 1-4.
  
  Joshi, R., Rama Prashat, Sharma, P. C., Singla-Pareek, S. L., & Pareek, A. (2016). Physiological characterization of gamma-ray induced mutant population of rice to facilitate biomass and yield improvement under salinity stress. Indian Journal of Plant Physiology, 21(4), 545-555. https://doi.org/10.1007/s40502-016-0264-x.
  
  Kebede, B., & Bobo, T. (2023). Demonstration of Rhodes grass (Chloris gayana Kunth) varieties at selected highland and midland agro-ecologies of Guji zone, Oromia, Ethiopia. Global Journal of Ecology, 8(2), 058-063. https://doi.org/10.17352/gje.000083.
  
  Kenana, R. S., Onjoro, P. A., & Ambula, M. K. (2020). Relative palatability and preference by red Maasai sheep offered brachiaria and Rhodes grass hay supplemented with calliandra leaves in Kenya. International Journal of Veterinary Science and Animal Husbandry, 5(5), 18-22.
  
  Khalifa, A. M., Abd-ElShafy, E., Abu-Khudir, R., & Gaafar, R. M. (2022). Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.). Journal of Genetic Engineering and Biotechnology, 20(166), 1-11. https://doi.org/10.1186/s43141-022-00424-2.
  
  Koryati, T., Ningsih, H., Erdiandini, I., Paulina, M., Firgiyanto, R., Junairah, & Sari, V. K. (2022). Pemuliaan tanaman [Plant breeding]. Yayasan Kita Menulis.
  
  Liu, R., Yang, X., Gao, R., Hou, X., Huo, L., Huang, Z., & Cornelissen, J. H. C. (2021). Allometry rather than abiotic drivers explains biomass allocation among leaves, stems and roots of Artemisia across a large environmental gradient in China. Journal of Ecology, 109, 1026-1040. https://doi.org/10.1111/1365-2745.13532.
  
  Makhziah, Sukendah, & Koentjoro, Y. (2017). Effect of gamma Cobalt-60 radiation to morphology and agronomic of three maize cultivar (Zea mays L.). Jurnal Ilmu Pertanian Indonesia, 22(1), 41-45. https://doi.org/10.18343/jipi.22.1.41.
  
  Marcu, D., Damian, G., Cosma, C., & Cristea, V. (2013). Gamma radiation effects on seed germination, growth and pigment content, and ESR study of induced free radicals in maize (Zea mays). Journal of Biological Physics, 39, 625-634. https://doi.org/10.1007/s10867-013-9322-z.
  
  Maryeni, R., Yusniawati, Yulfa D. & Chan, S. R. O. S. (2019). Genetic diversity based on RAPD marker of raime plants (Boehmeria nivea [L.] Gaud) in west Sumatera. IOP Conference Series: Earth and Environmental Science, 327, 012014. https://doi.org/10.1088/1755-1315/327/1/012014.
  
  Mganga, K. Z., Musimba, N. K. R., Nyariki, D. M., Nyangito, M. M., & Mwang’ombe, A. W. (2015). The choice of grass species to combat desertification in semi-arid rangelands is greatly influenced by their forage value for livestock. Grass and Forage Science, 70(1), 161-167. https://doi.org/10.1111/gfs.12089.
  
  Mohamed, A., & Gebeyew, K. (2018). On-farm performance evaluation of selected perennial grass under rain- fed conditions at Deghabour District, Cherer Zone, Ethiopian Somali Region. Poultry, Fisheries & Wildlife Sciences, 6(2), 1-5. https://doi.org/10.4172/2375-446x.1000202.
  
  Mohd Sharim, M. A., & Shamsiah, A. (2021). Detection of changes in growth, yield and genetic variation using RAPD markers among M1 V2 and M1 V3 generations of irradiated ginger (Zingiber officinale Roscoe). Food Research, 5(Suppl. 4), 74-82. https://doi.org/10.26656/fr.2017.5(S4).009.
  
  Mudhita, I. K., Putra, R. A., Rahman, M. M., Widyobroto, B. P., Agussalim, A., & Umami, N. (2024). The silage quality of Pennisetum purpureum cultivar Gamma Umami mixed with Calliandra calothyrsus and Lactiplantibacillus plantarum. Tropical Animal Science Journal, 47(1), 112-124. https://doi.org/10.5398/tasj.2024.47.1.112.
  
  Mulualem, T., Molla, M., & Yakob, T. (2012). Assessments of Alfalfa (Medicago sativa) and Rhodes grass (Chloris gayana) at Soddo and Kedidagamila districts of southern Ethiopia. Journal of Natural Sciences Research, 2(9), 30-36.
  
  Nei, M. (1987). Molecular evolutionary genetics. Columbia University Press.
  
  Nuraeni, Hernawati, Rani, S. R. A., Said, L. M., & Putri, A. A. (2023). Pertumbuhan tanaman kedelai (Glycine max L.) hasil radiasi sinar Gamma Cesium-137 [Growth of soybean (Glycine max L.) results from gamma radiation Cesium-137]. Journal Online of Physics, 8(3), 51-57. https://doi.org/10.22437/jop.v8i3.23715.
  
  Nurmansyah, Alghamdi, S. S., Migdadi, H. M., & Farooq, M. (2018). Morphological and chromosomal abnormalities in gamma radiation-induced mutagenized faba bean genotypes. International Journal of Radiation Biology, 94(2), 174-185. https://doi.org/10.1080/09553002.2018.1409913.
  
  Poerba, Y. S., Imelda, M., Wulansari, A., & Martanti, D. (2009). Induksi mutasi kultur in vitro Amorphophallus muelleri Blume dengan irradiasi gamma [In vitro cultural mutation induction of Amorphophallus muelleri Blume using gamma irradiation]. Jurnal Teknologi Lingkungan, 10(3), 355-364. https://doi.org/10.29122/jtl.v10i3.1482.
  
  Poorter, H., Niklas, K. J., Reich, P. B., Oleksyn, J., Poot, P., & Mommer, L. (2012). Biomass allocation to leaves, stems and roots: meta-analyses of interspecific variation and environmental control. New Phytologist, 193, 30-50. https://doi.org/10.1111/j.1469-8137.2011.03952.x.
  
  Pujiyanti, A. S., Wijaya, B. K., Artadana, I. B. M., Hardjo, P. H., & Purwanto., M. G. M. (2021). Character improvement of red rice (Oryza sativa L.) cv. Barak Cenana by mutagenesis using gamma irradiation. Jurnal Biologi Tropis, 21(2), 305-314. https://doi.org/10.29303/jbt.v21i2.2554.
  
  Raina, A., Laskar, R. A., Malik, S., Wani, M. R., Bhat, T. A., & Khan, S. (2021). Plant mutagenesis: Principle and application in crop improvement. In T. A. Bhat (Ed.), Mutagenesis cytotoxicity and crop improvement (pp. 38-65). Cambridge Scholars Publishing.
  
  Respati, A. N., Umami, N., & Hanim, C. (2018). Growth and production of Brachiaria brizantha cv. MG5 in three difference regrowth phase treated by Gamma radiation dose. Tropical Animal Science Journal, 41(3), 179-184. https://doi.org/10.5398/tasj.2018.41.3.179
  
  . Riviello-Flores, M. de la L., Cadena-Iñiguez, J., Ruiz-Posadas, L. D. M., Arévalo-Galarza, M. de L., Castillo-Juárez, I., Hernández, M. S., & Castillo-Martínez, C. R. (2022). Use of gamma radiation for the genetic improvement of underutilized plant varieties. Plants, 11, 1161. https://doi.org/10.3390/plants11091161.
  
  Rojas-Sandoval, J. (2020). Chloris gayana (Rhodes grass). CABI Compendium.
  
  Santosa, E., & Sugiyama, N. (2007). Growth and production of Amorphophallus paeoniifolius Dennst. Nicolson from different corm weights. Jurnal Agronomi Indonesia, 35(2), 81-87. https://doi.org/10.24831/jai.v35i2.1315.
  
  Santosa, E., Pramono, S., Mine, Y., & Sugiyama, N. (2014). Gamma irradiation on growth and development of Amorphophallus muelleri Blume. Jurnal Agronomi Indonesia, 42(2), 118-123. https://doi.org/10.24831/jai.v42i2.8428.
  
  Sarah, Nurcahyani, E., Handayani, T. T., & Mahfut. (2023). Respon pemberian ekstrak tauge (Vigna radiata (L.) R. Wilczek) pada medium murashige and skood terhadap pertumbuhan sawi hijau (Brassica rapa var. parachinensis L.) in vitro [Response to bean sprout extract Vigna radiata (L.) R. Wilczek) in murashige and. BIOMA: Jurnal Biologi Makassar, 8(2), 88-95. https://journal.unhas.ac.id/index.php/bioma.
  
  Setyawati, R., & Zubaidah S. (2021). Optimasi konsentrasi primer dan suhu annealing dalam mendeteksi gen leptin pada sapi Peranakan Ongole (PO) menggunakan Polymerase Chain Reaction (PCR) [Optimatization of primer concentration and annealing temperature in detecting leptin gene in Peranakan Ongole (PO) cattle using Polymerase Chain Reaction (PCR)]. Indonesian Journal of Laboratory, 4(1), 36-40. https://doi.org/10.22146/ijl.v4i1.65550.
  
  Singh, P. K., Sadhukhan, R., Kumar, V., & Sarkar, H. K. (2019). Gamma rays and EMS induced chlorophyll mutations in grasspea (Lathyrus sativus L.). International Journal of Bio-Resource and Stress Management, 10(2), 113-118. https://doi.org/10.23910/ijbsm/2019.10.2.1940b.
  
  Siregar, U. J., & Diputra, I. M. M. M. (2013). Keragaman genetik Pinus merkusii Jungh. et de Vriese strain tapanuli berdasarkan penanda mikrosatelit [Diversity of Pinus merkusii Jungh. et de Vriese of Tapanuli strain based on microsatellite markers]. Jurnal Silvikultur Tropika, 4(2), 88-99.
  
  Sulistyawati, P., & Widyatmoko, A. (2017). Keragaman genetik populasi kayu merah (Pterocarpus indicus Willd) menggunakan penanda Random Amplified Polymorphism DNA [Genetic diversity in Kayu merah (Pterocarpus indicus Willd) populations using random amplified polymorphism DNA marker]. Jurnal Pemuliaan Tanaman Hutan, 11(1), 67-76. https://doi.org/10.20886/jpth.2017.11.1.67-76.
  
  Tadesse, B., Tolemariam, T., & Hassen, W. (2022). Effect of different levels of biochar and inorganic fertilizer application on the growth of two grass species (Chloris gayana and Panicum coloratum). Ethiopian Journal of Applied Science and Technology, 13(1), 1-11.
  
  Tias, A. S. N., Moeljani, I. R., & Guniarti. (2022). Effect of gamma ray radiation 60Co generation M1 on growth and production of Cayenne pepper (Capsicum frutescens L.) prentul Kediri variety. In Nusantara Science and Technology Proceedings (pp. 84-92). Fakultas Pertanian UPN “Veteran” Jawa Timur. https://doi.org/10.11594/nstp.2022.2011.
  
  Toker, C., Yadav, S. S., & Solanki, I. S. (2007). Mutation breeding. In S. S. Yadav, D. L. McNeil & P. C. Stevenson (Eds.), Lentil an ancient crop for modern times (pp. 209-224). Springer.
  
  Tripathy, B. C., & Oelmüller, R. (2012). Reactive oxygen species generation and signaling in plants. Plant Signaling & Behaviour, 7(12), 1621-1633. https://doi.org/10.4161/psb.22455.
  
  Umami, N., Respati, A. N., Rahman, M. M., Umpuch, K., & Gondoe, T. (2022). Somatic embryogenesis and plant regeneration from the apical meristem of Wrukwona Napiergrass (Pennisetum purpureum) treated with thidiozuron and cupric sulfate. Tropical Animal Science Journal, 45(2), 220-226. https://doi.org/10.5398/tasj.2022.45.2.220.
  
  Umami, N., Widyobroto, B. P., Paradhipta, D. H. V., Solekhah, Z. A., & Nurjanah, L. L. (2023). Silage quality based on the physical and chemical of several napier grass varieties (Pennisetum purpureum) supplied with different levels of pollard. IOP Conference Series: Earth and Environmental Science, 118, 012015. https://doi.org/10.1088/1755-1315/1183/1/012015.
  
  Valenzuela, H., & Smith, J. (2002). Rhodesgrass. Sustainable agriculture cover crops, SA-CC-3. http://www2.ctahr.hawaii.edu/oc/freepubs/pdf/CoverCrops/rhodesgrass. pdf
  
  Warid, Khumaida, N., Purwito, A., & Syukur, M. (2017). Pengaruh iradiasi sinar gamma pada generasi pertama (M1) untuk mendapatkan genotipe unggul baru kedelai toleran kekeringan [The effect of gamma ray irradiation on the first generation (M1) to obtain a new superior genotype for drought tolerant soybeans]. Agrotrop, 7(1), 11-21.
  
  Wiryosimin, S. (1995). Mengenal asas proteksi radiasi [Getting to know the principles of radiation protection]. ITB Press.