PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

 

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Paralytic Shellfish Profiles Produced by the Toxic Dinoflagellate Pyrodinium bahamense from Sepanggar Bay, Malaysia

Asilah Al-Has, Normawaty Mohammad-Noor, Sitti Raehanah M. Shaleh, Mohd Nor Azman Ayub, Deny Susanti and Ghaffur Rahim Mustakim

Pertanika Journal of Tropical Agricultural Science, Volume 46, Issue 1, February 2023

DOI: https://doi.org/10.47836/pjtas.46.1.20

Keywords: Gonyautoxin, HPLC, PSP, Pyrodinium bahamense, saxitoxin

Published on: 22 Febuary 2023

Pyrodinium bahamense var compressum is a harmful dinoflagellate that produces saxitoxin, which causes paralytic shellfish poisoning (PSP) that is deadly to humans. A non-axenic culture of P. bahamense was established using f/2 media from samples collected from Sepanggar Bay, Kota Kinabalu, Sabah. Toxin analyses of cultures harvested on days 60, 120, 180, and 360 were performed using high-performance liquid chromatography with a fluorescence detector and compared with samples collected at the same location during the bloom in 2021. The highest cell toxin content was found in the bloom sample (86.2 fmole/cell), and no toxin was detected in the culture 60 days old. In addition, cell toxin content for the P. bahamense culture was low (9.4-16.5 fmole/cell). Based on the toxin profile, P. bahamense comprises 84- 98% of gonyautoxin 4. In summary, the current findings add to the existing knowledge of the toxin profiling of P. bahamense, a toxic, harmful algal bloom species, thus, leading to better toxin management.

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ISSN 1511-3701

e-ISSN 2231-8542

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JTAS-2538-2022

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