PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

 

e-ISSN 2231-8542
ISSN 1511-3701

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Vitrification of Blastocyst Murine Embryos Affects PI3K Pathway by Modulating the Expression of XIAP and S6K1 Proteins

MohdFazirul, M., Sharaniza, A. R., Norhazlin, J. M. Y., Wan Hafizah, W. J., Razif, D., Froemming, G. R. A., Agarwal A., Mastura, A. M. and Nor Ashikin, M. N. K.

Pertanika Journal of Tropical Agricultural Science, Volume 25, Issue S, August 2017

Keywords: Blastocyst, cryopreservation, embryo, PI3K, vitrification

Published on: 26 Feb 2018

Cryopreservation by vitrification has been widely used in Assisted Reproductive Technology (ART) to preserve embryos for an extended period of time. However, the effect of vitrification on development of the embryos is lacking. Therefore, understanding on vitrification effects on embryonic proteins, especially those involved in preimplantation development is crucial to provide high quality embryos for further usage. In this study, XIAP and S6K1 protein expressions following vitrification was investigated, since they have been implicated in diverse cellular processes including cell growth, migration, proliferation, differentiation, survival and development of preimplantation embryos via the PI3K pathway. Embryos were obtained from superovulated female ICR mice which were mated with fertile males. The embryos were harvested at the 2-cell stage and cultured until blastocyst stage. Blastocysts were then vitrified in ESF40 cryoprotectant. Western blot was carried out to determine the expression of XIAP and S6K1 proteins. The results showed the expression of XIAP and S6K1 significantly decreased in vitrified blastocyst compared to the control. This indicates that blastocyst vitrification may impact developmental competence through the activation of apoptotic pathways.

ISSN 1511-3701

e-ISSN 2231-8542

Article ID

JST-S0422-2017

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