PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

 

e-ISSN 2231-8542
ISSN 1511-3701

Home / Regular Issue / JTAS Vol. 27 (4) Oct. 2019 / JST-1411-2019

 

Efficacy of Silvernano Bacterial Cellulose Insole on Micrococcus sedentarius Growth

Kanchana Sitlaothaworn and Manussawee Dechkla

Pertanika Journal of Tropical Agricultural Science, Volume 27, Issue 4, October 2019

Keywords: Bacterial cellulose, foot odour, footpad, micrococcus sedentarius, silver nanoparticles

Published on: 21 October 2019

Silver nanoparticles (Ag0) impregnated into bacterial cellulose (BC) by immersion in an antimicrobial concentration of 1,700 µg/ml (10 mM) AgNO3 solution were used to synthesize silver nanoparticle-impregnated bacterial cellulose (Ag0BC) composites. Ag0BC incorporated into insertable footpad was developed for reduction of foot odor associated with pitted keratolysis, primarily caused by Micrococcus sedentarius. A scanning electron microscope (SEM) revealed the porous structure of Ag0BC fibers impregnated with Ag0 particles. The antibacterial activity of Ag0BC for Micrococcus sedentarius revealed a growth inhibition zone of 30 mm in diameter by disc diffusion method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of AgNO3 solution against Micrococcus sedentarius were 0.42 µg/ml and 0.83 µg/ml, respectively. The effficiency of an Ag0BC footpad applied to shoe samples was measured via total bacterial counts over a time period of 14 days. As expected, the percentage of surviving bacteria in three different types of shoe samples was gradually decreased in the period beginning on the 2nd day. Moreover, no bacterial growth was found in any shoe sample within 8–14 days. However, the cytotoxicity of 5.31–1,700 µg/ml AgNO3 concentrations revealed 50% growth inhibition (IC50) on human dermal fibroblast cells by resazurin microplate assay (REMA). Thus, it can be concluded that Ag0BC, synthesized with lower doses of AgNO3 concentration (< 1,700 µg/ml), may be able to apply in Ag0BC footpad production for inhibition of Micrococcus sedentarius growth and prevention of foot odour in different types of footwears.

ISSN 1511-3701

e-ISSN 2231-8542

Article ID

JST-1411-2019

Download Full Article PDF

Share this article

Recent Articles