A study was conducted to investigate the effects of EDTA and Chelex-100 on the glutamate dehydrogenase activity from a crude extract of the developing seeds of Lupinus angustifolius. The enzyme was not inhibited by Chelex-100; instead it was highly stimulated and its activity, when treated with Chelex-100, was higher than that of the control. Glutamate dehydrogenase, however, was inhibited by 5mM EDTA, stimulated at 30mM EDTA, and inhibited again at 45mM EDTA.