e-ISSN 2231-8542
ISSN 1511-3701
Nesti Fronika Sianipar and Ragapadmi Purnamaningsih
Pertanika Journal of Tropical Agricultural Science, Volume 41, Issue 1, February 2018
Keywords: Typhonium flagelliforme Lodd., new superior mutant, anticancer bioactive component, Indonesia
Published on: 21 Feb 2018
Rodent tuber (Typhonium flagelliforme Lodd.), which is a well known herbal plant from the Araceae family, is know for its anticancer activities. The genetic variation of rodent tuber is low due to its commonly applied vegetative propagation methods. Thus, its genetic variation has to be increased to obtain a new and superior plant containing a high amount of anticancer compounds. The aim of this study was to analyse the chemical compounds of the leaves and tubers of rodent tuber' mutant and non irradiated (control) plants by GC-MS method. In this study, in vitro calli of rodent tuber was irradiated with 6 Gy of gamma ray which produced mutant plantlets which was genetially different from non irradiated (control) plants. Mutant plantlets had been acclimated and propagated in the greenhouse to obtain the 6th generation vegetative mutant clones (MV6), which are stable superior mutants. The results indicated that MV6 contained six new anticancer compounds in the leaves and four new anticancer compounds in the tubers which have not been detected in control. The new anticancer compounds present in leaves and tubers were identified by GC-MS. They are hexadecanoic acid methyl ester, octadecadienoic acid, phytol¸ gamma-sitosterol, eicosane, geranylgeraniol, squalene, octacosane and 7-pentadecyne. MV6, is the new superior variety and a potential source of anticancer drugs.
ISSN 1511-3701
e-ISSN 2231-8542