The Colugo is a nocturnal arboreal mammal that inhabits the tropical rainforest in South East Asia. Photographs of the Colugo in gliding motion were taken using Digital SLR camera with the aid of speed light and flashlight to determine the Colugo sexes. These photographs technique could be used to determine the Colugo sexes without the need to capture the animal. It may also be a useful technique to determine the sexes of other gliding mammals and help in assisting the conservation effort of the mammal species.