e-ISSN 2231-8542
ISSN 1511-3701
Rafidah Saadun, Wen Siang Tan, Abdul Rahman Omar, Mohd. Hair Bejo, Majid Eshaghi, and Khatijah Yusoff
Pertanika Journal of Tropical Agricultural Science, Volume 31, Issue 1, February 2008
Keywords: Newcastle disease virus, infectious bursal disease virus, VP2 solubility, hypervariable region
Published on:
The hypervariable region (HVR) of VP2 protein of infectious bursal disease virus (IBDV) elicits neutralizing antibodies, but it is highly hydrophobic and tends to form inclusion bodies when expressed in Escherichia coli. To improve its solubility, the VP2(HVR) was fused to the C-terminal end of Newcastle disease virus (NDV) nucleocapsid (NP) protein and expressed in E. coli TOP 10 cells under the control of trc promoter. However, the fusion protein, NP-VP2(HVR)-trc, aggregated into insoluble inclusion bodies in the host cells. Therefore the coding region of NP-VP2(HVR) was sub-cloned into expression vectors containing the T7 promoter. The solubility of the NP-VP2(HVR)-T7 fusion proteins improved dramatically in E. coli BL21 (DE3), BL21 (SI) and Origami B cells.
ISSN 1511-3701
e-ISSN 2231-8542