Inquiry-based instruction is a student-centred approach that can enhance students' confidence, understanding, and academic performance in learning. This study seeks empirical evidence on the effect of Inquiry-Based Instruction on Active Learning and involves a sample of 147 undergraduate students in an institution of higher learning in Malaysia. Confirmatory Factor Analysis confirmed the second-order factor model of Inquiry-Based Instruction which consists of three latent constructs. On the other hand, the Active Learning construct was also represented as a second-order factor model with three latent constructs (Learning Obligation, Learning Effort and Learning Collaboration). Overall, Structural Equation Modeling results provided statistical evidence that Inquiry- Based Instruction has a strong, positive and significant effect on Active Learning in higher education.