Ramadhan is the fasting month in the Islamic calendar when consumption of water and food is abstained from. Previous studies of exercising during Ramadhan have shown mixed findings. The aim of this study was to determine the effects of a single bout of moderate cycling exercise during the ritual fasting of Ramadhan on mood states, rating of perceived exertion and short-term memory among physically inactive university students. Participants were 13 healthy male university students, with a mean age of 23.54±3.41 years, height of 170 ±7.02 cm, weight of 66.95±7.40 kg and body mass index (BMI) of 23.33±1.74 kg.m¯². Data on mood states, rating of perceived exertion and short-term memory were collected before and after cycling exercise. All of the participants performed cycling exercise at progressively increasing intensities of 45%, 60%, 75% of VO2max for 30 minutes (10 minutes for each intensity) a week before Ramadhan and in the first and fourth week of Ramadhan and 2 weeks after Ramadhan. The results of the mixed factorial ANOVA revealed no significant interactions between sessions across trials. In conclusion, exercise during Ramadhan fasting exhibited no significant effects on mood states, perceived exertion and cognitive performance.